Back to Services
Microsoft
Chai Discovery
Cradle
Escalante
Binding(includes Expression)
Price
from$99/protein
Speed
3-4 weeks
Output Data
Binding ClassificationKinetic ConstantsKinetic CurvesExpression Yield
Our Protein Binding workflow enables protein designers to easily validate protein binders by characterizing their interaction to one or several protein targets of interest. The assay is designed to be flexible in terms of the number of proteins and the number of concentrations to accommodate a wide range of applications. Run in Screening Mode, to quickly test new binders using 2 concentrations, and in Full Affinity Characterization, we perform a more detailed and precise analysis of the binding to a target using 4 or more concentrations.
Trusted byChai DiscoveryMicrosoftCradleEscalante
Chai DiscoveryMicrosoftCradleEscalante//
Protein types
You can test pretty much any type of protein, from small peptides, miniproteins, natural ligands, VHHs, scFvs, or Fabs. We are expressing thousands of proteins every month and see expression success rates of over 90%. For information on how to design your proteins so that they express well, check out our guide here.
//
Targets
We can work with nearly any soluble protein target that is commercially available, or we can custom express proteins for you. Browse our target catalog for an overview of available targets. If you need a custom or proprietary target, we can help source and prepare the appropriate materials.
//
Proprietary assay optimization and data processing
We've spent the last 2 years building the best binding data-generation pipeline with automated buffer optimization, non-specific binding detection, and custom fitting algorithms. You can trust us to get you the best data possible for your binding experiments.
Timeline
1
2
3
4
5
Day 0
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Day 1-7
Experiment Preparation
We design and order the DNA constructs and source and QC your target protein(s)
Day 8-9
Protein Expression
We design the DNA and express your proteins in our cell-free system
Day 10-15
Kinetic Measurements
We run all the binding assays and measure the kinetic curves
Day 16-21
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
1
Day 0
Designs Submission
Digitally upload your protein designs sequences on our secure platform
2
Day 1-7
Experiment Preparation
We design and order the DNA constructs and source and QC your target protein(s)
3
Day 8-9
Protein Expression
We design the DNA and express your proteins in our cell-free system
4
Day 10-15
Kinetic Measurements
We run all the binding assays and measure the kinetic curves
5
Day 16-21
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Deliverables
- Binding classification"Yes / No" binding classification
- Kinetic curvesFitted kinetic curves based on raw BLI/SPR data
- Kinetic constants*Precise KD, Kon, Koff. *only available if you opt for "Full affinity characterization"
- Expression yields"Low / Medium / High" yield classification
Binding Pricing
Configure your project and get an instant price estimate.
Assay Resolution
Do you mainly want to know if designs bind at all, or do you need precise kinetic constants?
Number of Proteins
Select how many proteins you want to characterize
244896192384768
Unavailable range due to lab workflow constraints
T2$169/sequence
Replicates
Select how many times to repeat each measurement
Duplicate included in price
Number of Targets
How many targets do you want to test against?
Experiment Layout
Cost Estimate
60 proteins × $169(T2)$10,140
Total$10,140
$169/protein
Delivery3-4 weeks
They validated their Binding with Adaptyv
MicrosoftValidating proteins generated by EvoDiff, a novel sequence-first protein design model.
Learn moreChai DiscoveryBenchmarking Chai-2, a zero-shot protein design model
Learn moreCradleOptimizing EGFR antibodies to improve affinity. Part of the Adaptyv EGFR Design Competition.
Learn moreEscalanteMulti-objective minibinders design and validation against PDL1 and IL7RA.
Learn moreFrequently Asked Questions
Browse step-by-step guides, references, and sample requirements.
Looking for more information? Take a look at our docs
or