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Expression
Price
from$49/protein
Speed
2-3 weeks
Output Data
Expression levelsSolubilityYield metrics
Our Expression workflow enables comprehensive analysis of protein expression levels across hundres or thousands of designed protein variants. Using high-throughput cell-free expression systems, we provide detailed characterization of the synthesis yield across different expression conditions.
Trusted by
Chai Discovery
Microsoft
Cradle
Escalante
Chai DiscoveryMicrosoftCradleEscalante//
Protein types
You can test pretty much any type of protein, from small peptides, miniproteins, natural ligands, or antibody formats such as Fabs. For information on how to design your proteins so that they express well, check out our guide here.
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Multiple expression systems
We currently offer E. coli- and Yeast-based cell-free expression systems. You can choose the system that best suits your protein or compare expression yields across both platforms.
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Expression screening and yield quantification
We offer two different ways to quantify the expression yield: a quick screening mode using our split-GFP method and a more detailed yield quantification mode using Capillary Electrophoresis and a Quant-iT assay.
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Run SEC on demand
Optionally, we can run SEC to further characterize the expressed proteins for monomeric or oligomeric states.
Timeline
1
2
3
4
5
Day 0
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Day 1-7
Experiment Preparation
We design and order the DNA constructs.
Day 8-9
Protein Expression
We express your proteins in our cell-free system and QC them
Day 10-12
Yield Quantification
We quantify the expression yield across different expression conditions
Day 13-14
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
1
Day 0
Designs Submission
Digitally upload your protein designs sequences on our secure platform
2
Day 1-7
Experiment Preparation
We design and order the DNA constructs.
3
Day 8-9
Protein Expression
We express your proteins in our cell-free system and QC them
4
Day 10-12
Yield Quantification
We quantify the expression yield across different expression conditions
5
Day 13-14
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Deliverables
- Split-GFP screeningRapid expression screening using split-GFP fluorescence readouts.
- Quantitative yield (CE/Quant-iT)Absolute yield quantification via capillary electrophoresis and Quant-iT assays.
- Expression QCQC of expressed proteins across systems and conditions.
- SEC analysis (optional)Size-exclusion chromatography to assess monomeric vs. oligomeric states.
Expression Pricing
Configure your project and get an instant price estimate.
Number of Proteins
Select how many proteins you want to characterize
244896192384768
Unavailable range due to lab workflow constraints
T2$79/sequence
Replicates
Select how many times to repeat each measurement
Duplicate included in price
Cost Estimate
60 proteins × $79(T2)$4,740
Total$4,740
$79/protein
Delivery2-3 weeks
Frequently Asked Questions
Browse step-by-step guides, references, and sample requirements.
Looking for more information? Take a look at our docs
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