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Thermostability(includes Expression)
Price
from$99/protein
Speed
2-3 weeks
Output Data
Melting curvesTm valuesAggregation data
Our Thermostability workflow allows you to characterize the thermostability of your protein variants and optimize their stability. Using nanoDSF, we measure at what temperature your protein designs start unfolding. We can test various buffer conditions, pH variants, salts, excipients, or ligand additions to evaluate their impact on Tm and aggregation. This data is essential for developing stable protein therapeutics, high-performing enzymes and robust proteins for diagnostic applications.
Trusted by
Chai Discovery
Microsoft
Cradle
Escalante
Chai DiscoveryMicrosoftCradleEscalante//
Supported protein formats
We support a wide range of protein formats for expression and thermostability analysis. Importantly, to be detected in the nanoDSF assay, proteins should contain a hydrophobic core with aromatic amino acids (such as tryptophan or tyrosine), as the assay relies on intrinsic fluorescence from these residues.
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High sensitivity thermostability assay
We use nanoDSF to measure the thermostability of your protein variants across a wide temperature range.
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Condition screening
Stability analysis across pH, salt, and buffer conditions.
Timeline
1
2
3
4
5
Day 0
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Day 1-7
Experiment Preparation
We design and order the DNA constructs for your protein variants
Day 8-9
Protein Expression
We express your proteins in our cell-free system, purify and QC them
Day 10-12
Assay Measurements
We run all the thermostability assays and measure the melting curves
Day 13-14
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
1
Day 0
Designs Submission
Digitally upload your protein designs sequences on our secure platform
2
Day 1-7
Experiment Preparation
We design and order the DNA constructs for your protein variants
3
Day 8-9
Protein Expression
We express your proteins in our cell-free system, purify and QC them
4
Day 10-12
Assay Measurements
We run all the thermostability assays and measure the melting curves
5
Day 13-14
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Deliverables
- Melting curvesThermal unfolding profiles upon temperature increase.
- Tm valuesMelting temperatures computed from the melting curves.
Thermostability Pricing
Configure your project and get an instant price estimate.
Number of Proteins
Select how many proteins you want to characterize
244896192384768
Unavailable range due to lab workflow constraints
T2$169/sequence
Replicates
Select how many times to repeat each measurement
Single included in price
Cost Estimate
60 proteins × $169(T2)$10,140
Total$10,140
$169/protein
Delivery2-3 weeks
Frequently Asked Questions
Browse step-by-step guides, references, and sample requirements.
Looking for more information? Take a look at our docs
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